Core Facility for Cryo-Electron Microscopy
INFORMATION
AFFILIATED ORGANIZATIONS
Charité Universitätsmedizin BerlinUSAGE POLICY
Click here to see usage policyLOCATION
Campus Berlin Buch (CBB)
DESCRIPTION
The Core Facility for cryo-Electron Microscopy (CFcryo-EM) is a dedicated facility for high resolution imaging of biological samples at cryogenic temperatures. Amongst other applications such as single particle data collection, our high-end instrumentation is suited to perform in situ structural biology by combining cryoCLEM, lamella FIB-milling, and cryo-electron tomography.
INFORMATION
The Core Facility cryo-EM provides advanced instrumentation for state-of-the-art three dimensional in-situ imaging of frozen hydrated biological samples. Specimen preservation by vitrification in liquid ethane can be done using a ThermoFisher Vitrobot Mark IV plunge freezer.
We use cryo electron tomography for three dimensional imaging at cryogenic temperatures using a ThermoFisher 300 keV Titan Krios TEM equipped with advanced direct electron detectors (K3 and Falcon III), energy filter, and Volta phase plate.
Thick samples like entire cells or tissue can be imaged after thinning by focused ion beam milling. Our facility provides aThermo Fisher Aquilos dual beam FIB/SEM to cut lamella suited to be imaged in the Titan Krios microscope.
Targeting areas of interest is guided by cryo-correlative light and electron microscopy (cryo-CLEM) using a LEICA SP8 confocal cryo light microscope.
We offer full service access to the instruments of the facility. The staff of CFcryoEM will be happy to assist you performing your own in-situ structural biology experiment. After training, all our devices can be operated independently by experienced users as well. Please contact us to discuss possibilities.
We use cryo electron tomography for three dimensional imaging at cryogenic temperatures using a ThermoFisher 300 keV Titan Krios TEM equipped with advanced direct electron detectors (K3 and Falcon III), energy filter, and Volta phase plate.
Thick samples like entire cells or tissue can be imaged after thinning by focused ion beam milling. Our facility provides aThermo Fisher Aquilos dual beam FIB/SEM to cut lamella suited to be imaged in the Titan Krios microscope.
Targeting areas of interest is guided by cryo-correlative light and electron microscopy (cryo-CLEM) using a LEICA SP8 confocal cryo light microscope.
We offer full service access to the instruments of the facility. The staff of CFcryoEM will be happy to assist you performing your own in-situ structural biology experiment. After training, all our devices can be operated independently by experienced users as well. Please contact us to discuss possibilities.
CONTACTS
christoph.diebolder@charite.de; cryo-em@charite.de